Endocrine Abstracts

Summary: Objective: To evaluate the expression level and binding affinity of glucocorticoid receptors (GRs)in patients with endogenous Cushing’s syndrome(CS) and to determine whether there is partial glucocorticoid resistance.Methods: Fifty-eight patients with CS and forty-one age- and sex-matched healthy volunteers were studied. The expression level and binding affinity of GRs in peripheral blood mononuclear cells (PBMLs) were examined by flow cyt...

Objective: To examine the relationship between C-peptide levels and endogenous insulin secretion and to understand the effects of elevated C-peptide after dosing on evaluating study insulin and its analogs’ PK/PD values in euglycemic clamps.Method: The study included 33 healthy male volunteers who underwent an 8-h euglycemic clamp with a subcutaneous injection of insulin aspart (0.2 U/kg). Blood glucose (BG), C-peptide, human insulin, and insu...

SYNGR3 is involved in the re-uptake of dopamine (DA) into striatal pre-synaptic terminals. Here we elucidate the genetic control of human SYNGR3 gene (SYNGR3).Methods and Intermediate Results: Step 1 Potential cis-acting in 2kb upstream of the transcription initiation site (TIS) of SYNGR3 were investigated using the MatInspector algorithm. Three potential Nurr1 binding sites were identified. Step 2 PCR amplification (human DNA as templa...